Validated in Performance, Accuracy and Quality
CCS has generated a set of recombinant cell lines stably expressing ion channels of high pharmacological relevance. The cell lines were optimized and validated by Cytocentrics, our competence partner for ion channel screening and patch-clamp analysis.
Cytocentrics supplies these cell lines as Frozen Instant Cells and provides custom patch-clamp screening services. Recombinant cell lines are available either on a full license basis or as ready-to-assay aliquots.
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HEK-hERG
hERG electrophysiology
Instant HEK 293 cells expressing the human ERG (Ether-a-go-go Related Gene) potassium chan-nel were characterized regarding their electrophysiological and pharmacological properties using conventional whole cell recordings. hERG currents were activated by depolarizing voltage steps beginning at –50 mV. Further depolarization led to a decrease in outward current amplitudes due to the onset of rapid inactivation.
Representative hERG outward currents were recorded from Instant HEK 293 cells stably expressing the hERG ion channel. hERG Currents were elicited upon depolarization of the membrane to +40 mV in 10 mV increments. hERG tail currents were elicited by means of a repolarizing step to -50 mV (2 s).
hERG Pharmacology
Dose response data for different compounds were obtained with conventional patch clamp. After whole cell establishment, increasing concentrations of quinidine, cisapride, E4031 and Terfena-dine were aplied to the cells.