Assay Ready Frozen Cells

The availability of cells at all times is a critical bottleneck in drug screening campaigns. Separating the production of cells from the actual screening process helps you to avoid this bottleneck and increases the flexibility of your screening schedule. Frozen Instant Cells which can be assayed without prior cultivation have become a valid and frequently used alternative to cells from a continuous growing culture. CCS was one of the first suppliers of Frozen Instant Cells for global pharmaceutical research. Years of experience makes CCS a reliable outsourcing partner, capable of fulfilling even your most challenging projects.

We are expanding cells under tightly controlled culture conditions to large bulk quantities. Cells are harvested applying a gentle method which does not affect surface receptors. Prepared to a density which corresponds to the requirements of your assay, the cells are dispensed into vials using an automatic filling device. This fast processing of the cells significantly decreases the time from harvest to freezing, which is critical for maintaining the quality of Frozen Instant Cells. Finally, the cells are frozen in a controlled rate freezer and stored at -150°C until they are shipped to you. To relive you from the storage of an entire batch, we can also provide deliveries on demand.

For secondary screening or drug profiling, in which you need only a few assay plates per day over a longer period of time, we can apply a proprietary technology to freeze cells directly in assay plates. Ready to use Princess® Instant Assays, can be stored at -80°C for an extended shelf life.
After thawing the required number of plates, you can perform the assay almost immediately.

All batches of cells and cell based products are carefully controlled for their quality before they are shipped to you. Frozen Instant Cells are checked not only for viability but also for their ability to adhere shortly after thawing. A good „Fitness“ of the cells is often correlated with fully functional response. Finally, the assay performance of the frozen cells is compared to cells from a continuous growing culture considering Z’, S/B and EC50.